anti-scd1 rabbit mab  (Bioss)

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Western Blot

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Primers of the genes used for qRT-PCR.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Sequencing

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Effects of BFA and TM on the mRNA and protein levels of apoptosis-related factors (PARP-1, caspase-12, caspase-3, and SCD1). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) PARP-1 , (B) caspase-12 , (C) caspase-3, and (D) SCD1 were determined by qRT-PCR. The protein levels of (E) cleaved PARP-1, (F) caspase-12, (G) full-length and cleaved caspase-3, and (H) SCD1 were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05 vs. control group; # P < 0.05 vs. DMSO (0.1% v/v, vehicle control) group; a P < 0.05 vs. BFA (0.25 mg/L) group. Abbreviations: BFA, brefeldin A; DMSO, dimethyl sulfoxide; PARP-1, poly (ADP-ribose) polymerase-1; qRT-PCR, quantitative real-time polymerase chain reaction; SCD1, stearoyl-CoA desaturase 1; SD, standard deviation; TM, tunicamycin.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Quantitative RT-PCR, Western Blot, Control, Real-time Polymerase Chain Reaction, Standard Deviation